Optimization of Formaldehyde Cross-Linking for Protein Interaction Analysis of Non-Tagged Integrinβ1
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چکیده
منابع مشابه
Optimization of Formaldehyde Cross-Linking for Protein Interaction Analysis of Non-Tagged Integrin β1
Formaldehyde cross-linking of protein complexes combined with immunoprecipitation and mass spectrometry analysis is a promising technique for analysing protein-protein interactions, including those of transient nature. Here we used integrin beta1 as a model to describe the application of formaldehyde cross-linking in detail, particularly focusing on the optimal parameters for cross-linking, the...
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ژورنال
عنوان ژورنال: Journal of Biomedicine and Biotechnology
سال: 2010
ISSN: 1110-7243,1110-7251
DOI: 10.1155/2010/927585